Process of preparing remedial substances from swine-blood.



GUSTAV LORENZ, on DARMSTADT, GERMANY, ASSIGNOR TO THE ROTHLAU'F sERUMGESELLSOHAFT MIT BESOHRANKTER I-IAFTUNG, on BERLIN, GER- MANY.

PROCESS OF PREPARING REMEDIAL SUBSTANCES FROM SWINE-BLOOD.

SPECIFICATION forming part of Letters Patent No. 645,l10, dated March13, 1900.

Application filed July 6,1898. Serial No. 685,267. o specimens.)

production of a permanent preparation con-.

taining the White corpusoles of the blood-serum of swine immunizedagainst swine fever, of which the following is a specification.

By a special treatment swine can be rendered immune against swinefeverin the following manner:

Immune swine have in the serum of their blood matters which wheninoculated in other swine are capable of causing the same to possess apassive immunity, as well as to effect a cure in cases in which theswine are already infected with swine fever. As the pure bloodserum, oreven that mixed with small quantities of disinfecting substances, canhardly be widely applied in veterinary practice, because it is tooliable to decomposition,while, on the other hand, it would render it tooexpensive to supply it in sterilized vessels and already divided intodoses in the manner in which diphtheritic-seru m antidote is prepared, Ihave directed my attention to the obtaining of a process for producing apreparation which shall possess the property of permanence and shall,moreover, have the advantage that it can always be brought to aparticular percentage of active material, which, in view of its multipleapplications, is of no small advantage.

The process discovered by me after considerable research and whichprovides the advantages above referred to is essentially as follows:From the fresh blood freed from its clot in the usual mechanical mannerand containing the white blood-corpuscles the serum is obtained byimmediate centrifugal separation. This serum is then purified fromcertain components which prevent the obtaining of the preparation inquestion. This purification is effected by adding first a small quantityof concentrated solution of chlorid of oaloium, and then by thedissolving in the mixture of a quantity of sulphate of ammonia soproportioned that a certain amount of slimy substances, of fattycompounds, and albuminous substances are separated,but no white Afterthese bodies have been.

of corpuscles. removed from the solution by filtering or simply byallowing them to coadhere the precipitation of the white corpuscles isefiected from the same. This body will be precipitated upon the additionof a further definite quantity of sulphate of ammonia, in combinationwith a series of albuminous bodies, and can be obtained by filteringthrough paper or other suitable filtering apparatus, as the precipitate,and can be purified by washing or resolution in water and repeatedpreciplita tion with sulphate of ammonia. After the substance thusobtained, which forms a white somewhat gray-green residue, has beendried upon earthen plates it is dissolved in a fluid compounded asfollows: Intwelve hundred parts of water ninety parts of salicylate ofsoda are dissolved and nine hundred parts of glycerin added to thesolution. This fluid is kept for use and poured separately over the dryresidue, after which there is added distilled water, five-per-cent.solution of car bonate of soda, and-five-per cent. solution of carbolicacid in equal parts, and in such quantity that the whole compound willcontain about five per cent. of carbolic acid and carbonate of sodarespectively. After the soluble parts are all dissolved the still cloudyfluid is separated from the specifically heavy insoluble compounds in acentrifugal apparatus, which has not theusual opening which is providedfor the removal of the specifically heavy parts, so that the saidinsoluble parts are deposited around the rim of the centrifugal drum asa kind of slime. The other insoluble substances consist of specificallylighter parts rising gradually to the top and forming here a white scum,while the fluid after a shorter or longer standing appears as a more orless clear solution which can be slowly let off by suitable devicesbelow the layers which cause the cloudiness. After the solution has beentested for its content of white corpuscles by experiments on livinganimals the said solution can be made normal by the further addition ofany of the above-mentioned fluids in the event that there is anydeficiency in any of these in the original mixing.

precipitate will include only the fatty compounds and albuminoussubstances, which interfere with the subsequent solution of the residuecontaining the white corpuscles, and then precipitating the whitecorpuscles by the further addition of sulphate of ammonia.

2. The method of preparing a permanent preparation containing indetermined proportions the White corpuscles of the blood of swineimmuned again-st swine fever consisting in purifying the serum with aconcentrated'solution of chlorid of calcium, allowing the serum to standa short time, then precipitating the same fractionally with sulphate ofammonia in such proportions that the first precipitate will include onlythe fatty compounds and albuminous substances, which interfere with thesubsequent solution of the residues containing the White corpuscles, andthen precipitating the white corpuscles by the further addition ofsulphate of ammonia, drying the precipitate containing the Whitecorpuscles upon plates and then dissolving the same in a fluid composedof Water, glycerin, salicylate of soda, carbonate of soda, and carbolicacid in the proportions substan tially as described. I

In witness whereofIhave hereunto set my hand in presence of twoWitnesses.

GUSTAV LORENZ, Witnesses:

PAUL SoHU'LZ, ANTON LIPPERT.

